Science Museum is located on the Line 3 (Line 3), one of Mumbai's operational metro corridors. It sits between Acharya Atre Chowk and Mahalaxmi, making it a convenient stop for the surrounding area.

The station layout follows the standard Mumbai Metro design – entry gates at street level, ticket counters and machines in the concourse, and platforms accessible via escalators, stairs, or lifts.

The area around Science Museum station offers good connectivity to local landmarks. Nehru Science Centre is about a 5-minute walk from the exit.